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Ion-Exchange Chromatography: Basic Principles and Application. Philip M. Cummins, Keith D. Rochfort & Brendan F. O'Connor. Protocol. First Online: 12 October …
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Besides the use of adsorption models, ion exchange systems may be more correctly represented by the mass action law (MAL). This is the most characteristic property of ion exchange and can be used as one of the possible modeling equations. Actually, it expresses the typical double exchange reaction where the balancing ion in the …
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Ion-exchange resins (IRA-410, D201, D301, D900, D392R, JK206, D202, and D296) were obtained from Langfang Sennart Chemical Company, and their basic properties are listed in Table S3. The resins were used after pretreatment. 4.2. Preparation of BHET and r-PET.
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Ion-exchange reaction, any of a class of chemical reactions between two substances (each consisting of positively and negatively charged species called ions) that involves an exchange of one or more ionic components. …
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In literature, the terms "ion exchange chromatography" and " ion chromatography " (IC) are often used interchangeably, thanks to the wide use of IEX, and refer to the separation of polar or ionic species. However, IC is really an umbrella term that includes IEX along with ion exclusion chromatography (IEC) and ion pair (IP) …
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Ion exchange can be used for wine, fruit juice, and whey demineralization, and cane sugar decolorization. In whey demineralization, ion exchange is used to recover and convert lactose and mineral salts …
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Share. We explain the occurrence of ion exchange and an index profile around the focal point inside a commercial crown glass formed by femtosecond laser irradiation. The index profile in the photoinduced area has a ring-shaped pattern, which indicates that local densification occurred in the glass. An irregular surface reflecting the …
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The mechanism of lithium ion-sieve adsorbing Li+ ions from brines is based on the Li+/H+ ion exchange, where Li+ ions in brines are adsorbed on ion-sieves to displace H+ ions, and the displaced H+ ions are released in brines in turn. If the released H+ ions are accumulated in brines, then brines become acidic, not conducive to Li+ ions …
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Ion exchange chromatography is the reversible adsorption of charged molecules to immobilized ion groups on a matrix of an opposite charge. Separation can be selectively …
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Applications of ion exchange In the laboratory. Ion exchange is used for both analytical and preparative purposes in the laboratory, the analytical uses being the more common. An important use of ion-exchange chromatography is in the routine analysis of amino acid mixtures. Columns of cation-exchange resin are used, and the solutions are maintained …
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An anion-exchange resin (Dowex 1×8) and a cation-exchange resin (Dowex 50W×8) were used for the determination of the distribution coefficients (Kd) for the following elements: Sr, Cd, Ba, Ra, Sc, Y, Pm, Lu, Ac, Ti, Zr, Hf, Th, As, Sb, and Se in acetic acid media. Concentration of acetic acid was expanded down to 0.01 M and the distribution …
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Step 4. Using the 10 mL graduated cylinder add some amount of RO water to the sample of green salt and gently swirl the beaker until the unknown salt is completely dissolved. Then, place a clean 150 mL beaker under the ion exchange column and quantitatively transfer the solution of green salt to the column. Collect the eluate in the 150 mL beaker.
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More rarely the term "high performance ion-chromatography− mass spectrometry" (HPIC-MS) is also used. In this review we will use the term IC-MS to refer to the analytical platform and the "ion-exchange chromatography−mass spectrometry" sepa-ration mode, unless otherwise stated, in line with common usage in the literature.
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A second sub-category of liquid chromatography is known as ion-exchange chromatography. This technique is used to analyze ionic substances. It is often used for inorganic anions (e.g., chloride, nitrate, and sulfate) and inorganic cations (e.g., lithium, …
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Treatment of PFAS with anion exchange resins has been demonstrated in numerous small and large scale applications. The following collection is a sample of published examples for PFAS removal using ion exchange resin. These examples also highlight potential system providers in the marketplace.
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Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad …
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Ion-exchange chromatography (IEX) separates proteins (or any biomolecules) based on differences in their net charge at a particular pH. Protein charge depends on the number and type of ionizable amino acid side chain groups. Each protein has an isoelectric point (pI), the pH at which the overall number of negative and positive …
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WQ1031 Water Softening (Ion Exchange) This publication explains how softeners work, what to ask when purchasing a softener and the health and aesthetic benefits and or detriments. Lead Author: Tom Scherer, Ph.D., Extension Agricultural Engineer, North Dakota State University. Availability: Web only. Mg/l. or ppm. Grains per Gallon (gpg)
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The Ion Exchange Process. Softening is the process of ion exchange. The ions in hard minerals (calcium and magnesium) are exchanged with ions of "soft" minerals such as sodium, which do not produce the hard scale that can damage foodservice equipment. Water softener systems consist of two tanks: a resin tank and a brine tank.
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Ion exchange chromatography, or IEX, is a class of liquid chromatography (LC) used to separate organic and inorganic molecules. One area in which it is particularly useful, and the focus of this article, is …
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With the use of an anion exchange column instead of a cation exchange it became possible to achieve on-line 2D chromatography implementation (IEx – RP). Additionally, we used a two-step chromatographic procedure, a combination of cation exchange purification followed by anion exchange chromatography that allowed for isolating a C-peptide ...
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There are two general types of ion exchange resins: those that exchange positive ions, called cation exchange resins, and those that exchange negative ions, called anion exchange resins. A cation is an ion with a positive charge. Common cations include Ca +2, Mg +2, Fe +2, and H +1. A cation resin is one that exchanges positive ions.
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The ion-exchange process in silicate glass dissolution. The ion-exchange process during silicate glass dissolution is mainly proton (H +)—alkali ion, e.g., sodium (Na +) exchange.The process can ...
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In anion exchange chromatography, the exchanging ions are anions and the equation is represented as follow; S-X+A- + B- ↔ S-X+B-+ A- S-X + A - + B - ↔ S-X + B - + A -. The anion B - of the eluent replaced with the analyte cation A - bound to the positively charged ion X + on the surface of the stationary phase.
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Ion-exchange chromatography is just one of many separation techniques used to purify proteins [1] and in this article, we will cover its basic principles, applications, and how to optimize this important …
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2.1.4 Ion Exchange Systems. Ion exchange resins consists of two main types, i.e., cation exchange resins, that exchange positively charged ions, such as sodium, for calcium, and anion exchange resins, that exchange negatively charged ions, such as chloride, for arsenic. Resins are usually made from the polystyrene polymer backbone and differ ...
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This will be done using two independent methods found in this experiment and the next experiment. The lab manual presents background and procedural information first for the …
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In order to mitigate these issues and enhance the efficacy of your ion exchange system, consider using pre-treatment processes like chemicals or filtration. The industry for ion exchange systems is a booming one, which is mainly caused by the need for an effective ion exchange resin. In 2014, this industry brought in around $1.54 billion.
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The ability of ion-exchange resin for ammonia removal from aqueous solution was studied. The results showed that Amberlite ion-exchange resin was effective in removing ammonia from aqueous solution. Factorial design and response surface methodology were applied to evaluate and optimize the effects of pH, resin dose, contact …
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Q Can a cation exchange resin be used to separate protein isomers having pI between 5.6–6.15? A Yes, if the isomers differ in charge by this small amount, you should use a high resolution ion-exchange resin (e.g., MonoBeads™ columns) and apply low amounts of protein and run long gradients. However, you will probably
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