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The main reasons that traditional refrigerants cause global warming are the emissions of CO 2 and fluorocarbons [1,2].Liquid nitrogen, as a cooling medium with low boiling point, large latent heat, high cooling rate, and high development value, is widely used in low-temperature environment simulations such as biomedicine, food freezing, and low …
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Nitrogen is expected to be an effective method to extinguish large-scale fires and suppress explosions. The key to its ing process is that gas nitrogen (N2) and a solution are mixed uniformly in a generator. Moreover, liquid nitrogen (LN2) has been proposed as a source of gas nitrogen to generate nitrogen …
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This study aimed to evaluate the effects of different liquid nitrogen freezing (LNF) temperatures (-20, −40, −60, −80, and −100 °C) on the water holding capacity, texture, microstructure, and flavor of Crassostrea gigas (C. gigas).The results showed that −40 °C LNF, −60 °C LNF, and −80 °C LNF improved the water holding capacity of C. …
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The availability of bioresources is a precondition for life science research, medical applications, and diagnostics, but requires a dedicated quality management to guarantee reliable and safe storage. Anecdotal reports of bacterial isolates and sample contamination indicate that organisms may persist in liquid nitrogen (LN) storage tanks. …
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Show abstract. PDF | This protocol permits the simultaneous extraction of clean DNA from many samples with little reagent waste, thus decreasing the cost of …
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Biology Reporter, 20: 299a−299e. Introduction Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare the tissues for …
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Plant DNA Extraction Protocol Source: Protocol modified from Keb-Llanes et al. (2002) Plant Molecular Biology Reporter, 20: 299a−299e. Introduction Plant materials are among the most difficult for high quality DNA extractions. The key is …
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The tissue was ground into a fine powder in liquid nitrogen and transferred with a cold microspatula to a 1.5 mL microtube. ... (29 and 116 µg g −1 of fresh weight, ... Plant Mol Biol Rep 20:299a–299e. Article CAS Google Scholar Cota-Sánchez JH, Remarchuk K, Ubayasena K (2006) Ready to use DNA extracted with a CTAB method …
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Source: Protocol modified from Keb-Llanes et al. (2002) Plant Molecular BiologyPlant materials are among the most difficult for high quality DNA extractions. The key is
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It was stored at − 80 °C till further use. ... Briefly, fresh leaves (0.5 g) was taken and ground to fine powder (using pre-chilled mortar pestle) using liquid nitrogen and 1.5 ml of pre-warmed (65 °C) CTAB buffer was added and transferred to two eppendorf tubes and mixed well by inverting the tubes and incubated at 60 °C for 1 h in ...
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Bulk and Prepack available | Aldrich-295574; Nitrogen ≥99.998%; CAS No. ; Explore related products, MSDS, application guides, procedures and protocols at Sigma Aldrich - a one stop solution for all your research & industrial needs.
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Reporter, 20: 299a−299e. Introduction Plant materials are among the most difficult for high quality DNA extractions. …
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In China, the capacity to produce coalbed methane and extract underground gas is restricted by the prevalence of low-permeability coal seams. Liquid nitrogen fracturing is a new low temperature–high-pressure anhydrous …
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The temperature of liquid nitrogen is −195.79 °C (77 K; −320 °F). Liquid nitrogen has many uses, but poses risks of frostbite, explosion, and suffocation if handled incorrectly. Liquid nitrogen is very …
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The evaporation process of a liquid nitrogen droplet in different immiscible superheated bulk fluids has been quantified experimentally for the first time. The initial liquid nitrogen droplet size was found to have the dominant impact on the consequential evaporation rate. A scaling law based on the diffusion-controlled droplet evaporation ...
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It has good temperature sensitivity, with the test sensitivity reaching 226.7 μV °C −1. Most importantly, it can work in a wide temperature range, from extremely low temperatures down to liquid nitrogen temperature to high temperatures up to 1200 °C, which is difficult to be achieved by other existing flexible temperature sensors.
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Abstract Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One …
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On a small scale, pure nitrogen is made by heating. Nitrogen, nonmetallic element of Group 15 [Va] of the periodic table. It is a colorless, odorless, tasteless gas that is the most plentiful element in Earth's atmosphere and is a constituent of all living matter. Its atomic number is 7 and it is denoted by the symbol 'N' in the periodic ...
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To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N 2 vapour phase or in liquid nitrogen …
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Visit ChemicalBook To find more Nitrogen() information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. You can also browse global suppliers,vendor,prices,Price,manufacturers of Nitrogen(). At …
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Most pure nitrogen comes from the fractional distillation of liquid air. The atmosphere consists of 78% nitrogen by volume. This means there are more than 20 million tons of nitrogen over every square mile of the earth's surface. Nitrogen is a component of proteins and of the genetic material (DNA/RNA) of all plants and animals.
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1. Introduction. Liquid nitrogen (LN 2) spray is extensively used in modern industries, e.g., medical refrigeration [1], equipment cooling [2], food preservation [3], and wind tunnel refrigeration [4].LN 2 spray is a gas–liquid two-phase jet composed of ambient gas, nitrogen, and droplets. The evaporation of single moving droplet is a fundamental …
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299a−299e. Introduction Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare the tissues for extraction. In ... liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethyl
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The primer set KZ-AQ-3F/KZ-AQ-3R was selected for its specificity (amplifies only K. zonata) and sensitivity (1 × 10−5 ng/µL detection). This is the first report of a …
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An exploratory study on enhancing coal porosity/permeability using freeze−thaw cycling with liquid nitrogen (LN 2) was conducted. The changes of fracture and porosity in coal with the freeze−thaw treatment using LN 2 were evaluated using nuclear magnetic resonance (NMR). After freeze−thaw treatment, the coal pore size tended to increase ...
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299a−299e. Introduction Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare the tissues for extraction. In most cases this …
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The primer set KZ-AQ-3F/KZ-AQ-3R was selected for its specificity (amplifies only K. zonata) and sensitivity (1 × 10−5 ng/µL detection). This is the first report of a Kretzschmaria zonata ...
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Fig. 299e-f Two parts of the same original spinel are ... Fig. 302a-b UV-VIS-near IR absorption spectra recorded at liquid nitrogen ... which may play a role of O 2− → Fe 2+ and O 2− → Fe ...
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The standard free-energy change can be calculated from the definition of free energy, if the standard enthalpy and entropy changes are known, using Equation 18.8.7: ΔG° = ΔH° − TΔS°. If ΔS° and ΔH° for a reaction have the same sign, then the sign of ΔG° depends on the relative magnitudes of the ΔH° and TΔS° terms.
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Background A variety of basic and applied research programs in plant biology require the accurate and reliable determination of plant tissue cold hardiness. Over the past 50 years, the electrolyte leakage method has emerged as a popular and practical method for quantifying the amount of damage inflicted on plant tissue by exposure to …
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